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Topic C.2 · SL and HL

Cell Respiration: notes and practice questions

Summary
  • This topic explores how cells release energy from carbon compounds to produce adenosine triphosphate (ATP).
  • ATP is the energy currency of cells, used for processes like active transport, macromolecule synthesis, and cellular movement.
  • Energy is released when ATP is hydrolysed to ADP and phosphate, and required to synthesize ATP from ADP and phosphate.
  • Cell respiration uses carbon compounds like glucose and fatty acids as substrates to produce ATP.
  • Aerobic respiration requires oxygen, occurs in mitochondria, and yields more ATP than anaerobic respiration.
  • Anaerobic respiration in humans produces lactate; simple word equations for both types, with glucose as substrate, should be known.
  • The rate of cell respiration can be measured and calculated.

How it is examined

At SL the word equations and the aerobic/anaerobic comparison are the two reliable items, and the comparison is usually a table in the mark scheme. At HL, the four stages and where each happens (cytoplasm, matrix, inner membrane) are asked repeatedly, and location marks are awarded separately from process marks. Naming an intermediate other than citrate or oxaloacetate earns nothing but costs nothing. Rate-of-respiration calculations from respirometer data show up in Paper 1B.

Key ideas
  • C1.2.1 ATP as the molecule that distributes energy within cells. Include the full name adenosine triphosphate and that it is a nucleotide, and the properties that make it suitable as the energy currency of the cell.
  • C1.2.2 Life processes that ATP supplies with energy: active transport across membranes, synthesis of macromolecules (anabolism), and movement of the whole cell or of cell components such as chromosomes.
  • C1.2.3 Energy transfers during interconversion between ATP and ADP. Energy is released by hydrolysis of ATP to ADP and phosphate; energy is required to synthesize ATP from ADP and phosphate.
  • C1.2.4 Cell respiration as a system for producing ATP within the cell using energy released from carbon compounds. Glucose and fatty acids are the principal substrates but a wide range of carbon compounds can be used. Students should be able to distinguish cell respiration from gas exchange.
At HL
  • C1.2.7 Role of NAD as a carrier of hydrogen, and oxidation by removal of hydrogen. Oxidation is electron loss, so removing hydrogen with its electron (dehydrogenation) oxidizes the substrate. Redox reactions involve both oxidation and reduction, and NAD is reduced when it accepts hydrogen.
  • C1.2.8 Conversion of glucose to pyruvate by stepwise reactions in glycolysis with a net yield of ATP and reduced NAD. Include phosphorylation, lysis, oxidation and ATP formation.
  • C1.2.9 Conversion of pyruvate to lactate as a way of regenerating NAD in anaerobic cell respiration, which allows glycolysis to continue with a net yield of two ATP per glucose.
  • C1.2.10 Anaerobic cell respiration in yeast and its use in brewing and baking. The pathways are the same in humans and yeast apart from how NAD is regenerated using pyruvate, and therefore the final products differ.

Guiding questions

  • What are the roles of hydrogen and oxygen in the release of energy in cells?
  • How is energy distributed and used inside cells?

Linking questions

  • In what forms is energy stored in living organisms?
  • What are the consequences of respiration for ecosystems?

Practice questions

12 questions · 4 easy · 7 medium · 1 hard
Showing 12 of 12

Question 1

EasyPaper 1A · calculator1 mark

During intense exercise, human muscle cells may not receive enough oxygen. Which products are formed from glucose during anaerobic respiration in these cells?

A. Ethanol and carbon dioxide

B. Lactate only

C. Lactate and ATP

D. Carbon dioxide and water

Question 2

MediumPaper 2 · calculator12 marks
(a)

Thermoregulation is a key homeostatic process. A study investigated the response of human participants to a cold environment (10∘C10^\circ\text{C}). Participants were divided into two groups: one group remained at rest, while the other performed moderate exercise on a stationary bicycle. Core body temperature was measured every 15 minutes for two hours.

![Line graph showing core body temperature (°C)

over 120 minutes for two groups: Resting and Exercising. The y-axis is Core Body Temperature (°C) from 36.5 to 37.5. The x-axis is Time (minutes) from 0 to 120. Both groups start at 37.0°C. The Resting line shows a steady decline to about 36.6°C. The Exercising line shows a very slight initial dip, then rises to about 37.2°C and remains stable.](https://jujwjkjdeuwpscvsbilk.supabase.co/storage/v1/object/public/question-images/b242d1e2-5cff-4548-9da6-249648b0b274/1791279084335.png){width="3.6in"}

(a) Describe the trend in core body temperature for the exercising group.

[2]
(b)

(b) Compare the core body temperature in the resting group with the exercising group over the 120-minute period.

[2]
(c)

(c) Suggest a reason for the difference in core body temperature observed between the two groups.

[1]
(d)

(d) The resting group then underwent a 10-week cold acclimatization program, involving regular, controlled exposure to cold. Their core body temperature response to the same cold environment (10∘C10^\circ\text{C}) at rest was measured again.

![Line graph comparing core body temperature (°C)

over 120 minutes for the same group Pre-acclimatization and Post-acclimatization. The y-axis is Core Body Temperature (°C) from 36.5 to 37.5. The x-axis is Time (minutes) from 0 to 120. Both lines start at 37.0°C. The Pre-acclimatization line shows a steady decline to 36.6°C. The Post-acclimatization line shows a much smaller decline, stabilizing around 36.9°C.](https://jujwjkjdeuwpscvsbilk.supabase.co/storage/v1/object/public/question-images/b242d1e2-5cff-4548-9da6-249648b0b274/1791279066631.png){width="3.6in"}

Distinguish between the results pre- and post-acclimatization.

[2]
(e)

(e) Suggest why participants were required to rest in a thermoneutral room (24∘C24^\circ\text{C}) for 30 minutes before each experimental trial.

[1]
(f)

(f) The study also measured the resting metabolic rate of the participants before and after the acclimatization program.

![Bar chart showing Resting Metabolic Rate (mL O2 kg⁻¹ min⁻¹)

Pre-acclimatization and Post-acclimatization. The y-axis is Resting Metabolic Rate. The Pre-acclimatization bar is lower than the Post-acclimatization bar. Error bars are shown.](https://jujwjkjdeuwpscvsbilk.supabase.co/storage/v1/object/public/question-images/b242d1e2-5cff-4548-9da6-249648b0b274/1791279048041.png){width="3.6in"}

Describe the difference in resting metabolic rate before and after the program.

[1]
(g)

(g) Suggest how an increased resting metabolic rate might contribute to the improved tolerance to cold shown in part (d).

[1]
(h)

(h) The hormone thyroxin is involved in regulating metabolic rate. Outline the role of thyroxin in thermoregulation.

[2]

Question 3

HardPaper 1B · calculator6 marks
(a)

The graph shows data-logging of three variables in a coastal rock pool containing the brown alga Fucus vesiculosus over a 48-hour period.

A graph showing changes over 48 hours in a rock pool. The x-axis is time from 0 to 48 hours. There are three y-axes: pH (ranging from 7.4 to 8.6), light intensity in $\text{W m}^{-2}$ (ranging from 0 to 1000), and temperature in °C (ranging from 10 to 30). On day 1, light intensity peaks at $1000\text{ W m}^{-2}$ at 12 hours, temperature peaks at 25 °C at 14 hours, and pH peaks at 8.5 at 13 hours. During the night (18 to 30 hours), light is 0, temperature drops to 15 °C, and pH drops to 7.5. On day 2, at 36 hours, light intensity drops sharply to $200\text{ W m}^{-2}$ due to fog, but temperature remains high at 24 °C. During this fog period, the pH drops to 7.7.

Explain the causes of the rises and falls in the pH of the rock pool water.

[2]
(b)(i)

(b.i) State the relationship shown in the graph between light intensity and dissolved CO2\text{CO}_2 concentration.

[1]
(b)(ii)

(b.ii) State the relationship shown in the graph between temperature and dissolved CO2\text{CO}_2 concentration.

[1]
(c)

(c) From the graph, deduce whether light intensity or temperature has a greater effect on the dissolved CO2\text{CO}_2 concentration.

[2]

Question 4

EasyPaper 1A · calculator1 mark

Which statement correctly compares aerobic and anaerobic cell respiration in humans?

A. Aerobic respiration produces lactate and a small yield of ATP.

B. Anaerobic respiration occurs in the mitochondria and produces a large yield of ATP.

C. Aerobic respiration requires oxygen and produces a larger yield of ATP than anaerobic respiration.

D. Anaerobic respiration produces carbon dioxide and water.

Question 5

MediumPaper 1B · calculator10 marks
(a)

(a) The table shows the areas of different membranes (μm2\mu\text{m}^2) in a plasma cell (an antibody-secreting B lymphocyte).

MembraneArea / μm2\mu\text{m}^2
Rough endoplasmic reticulum18 000
Smooth endoplasmic reticulum150
Outer mitochondrial membrane1 200
Inner mitochondrial membrane6 500
Plasma membrane864
Golgi apparatus1 800
Nucleus300

Calculate the volume of the plasma cell, assuming it is cubic.

[3]
(b)

(b) Explain the difference in area of the inner and outer mitochondrial membranes.

[3]
(c)

(c) Justify the conclusion that the plasma cell is highly specialized for protein secretion.

[2]
(d)

(d) Comment on the area of the smooth endoplasmic reticulum in the plasma cell.

[2]

Question 6

EasyPaper 1A · calculator1 mark

The diagram shows the structure of a mitochondrion. Where does the Krebs cycle occur?

Diagram of a mitochondrion. A points to the outer membrane. B points to the inner membrane (cristae). C points to the matrix. D points to the intermembrane space.

A. A

B. B

C. C

D. D

Question 7

MediumPaper 2 · calculator10 marks
(a)(i)

(a.i) Ruminococcus albus is a bacterium found in the rumen (first stomach) of cattle. It breaks down cellulose from ingested grass and produces acetate (CH3COO−\text{CH}_3\text{COO}^-) as a waste product.

Identify, with a reason, whether R. albus is autotrophic, heterotrophic or mixotrophic.

[2]
(a)(ii)

(a.ii) Identify, with a reason, whether R. albus is holozoic or saprotrophic.

[2]
(b)

(b) R. albus is difficult to study in standard laboratory conditions because it dies rapidly when exposed to air. Explain two conclusions that can be drawn from this observation.

[4]
(c)

(c) The epithelial cells of the cow's rumen absorb the acetate produced by the bacteria. Suggest how the cow's cells might use this substance.

[2]

Question 8

EasyPaper 1A · calculator1 mark

Human muscle cells can generate ATP through both aerobic and anaerobic respiration depending on the availability of oxygen. Which row correctly identifies the products of these two pathways when glucose is the substrate?

Aerobic respirationAnaerobic respiration
A.Carbon dioxide and waterLactate
B.Carbon dioxide and waterEthanol and carbon dioxide
C.Carbon dioxide onlyLactate
D.Carbon dioxide onlyEthanol and carbon dioxide

Question 9

MediumPaper 1B · calculator9 marks
(a)

(a) The graph shows the results of an experiment in which the oxygen consumption rate of deer mice (Peromyscus maniculatus) was measured at various environmental temperatures from −5 ∘C-5~^\circ\text{C} to 35 ∘C35~^\circ\text{C}. Between −5 ∘C-5~^\circ\text{C} and 30 ∘C30~^\circ\text{C}, the mice maintained a constant core body temperature, but above 30 ∘C30~^\circ\text{C} their body temperature increased.

Scatter plot with error bars showing oxygen consumption rate in $\text{cm}^3 \text{ O}_2 \text{ g}^{-1} \text{ h}^{-1}$ on the y-axis against environmental temperature in $^\circ\text{C}$ on the x-axis ranging from $-5$ to $35$. Oxygen consumption is highest at $-5~^\circ\text{C}$, decreases linearly to a stable plateau between $20~^\circ\text{C}$ and $30~^\circ\text{C}$, and rises again at $35~^\circ\text{C}$.

Describe the relationship between environmental temperature and oxygen consumption rate in the deer mice.

[3]
(b)

(b) Explain the change in oxygen consumption rate as the environmental temperature drops from 20 ∘C20~^\circ\text{C} to −5 ∘C-5~^\circ\text{C}.

[3]
(c)

(c) Suggest a reason for the change in oxygen consumption rate as environmental temperatures increased from 30 ∘C30~^\circ\text{C} to 35 ∘C35~^\circ\text{C}.

[1]
(d)

(d) Suggest two reasons for the variation in oxygen consumption rate between individual mice at each environmental temperature.

[2]

Question 10

MediumPaper 1A · calculator1 mark

An influx of agricultural fertilizer into a lake causes a rapid increase in the population of algae. Following the death of the algae, the dissolved oxygen concentration in the lake drops significantly.

Which process is directly responsible for this decrease in oxygen?

A. Aerobic respiration by saprotrophic bacteria

B. Cessation of photosynthesis by the dead algae

C. Anaerobic respiration by decomposers

D. Aerobic respiration by primary consumers

Question 11

MediumPaper 1A · calculator1 mark

During a high-intensity sprint, human muscle cells rely heavily on anaerobic respiration. Which row correctly identifies the word equation for this process and its ATP yield compared to aerobic respiration?

Word equationATP yield compared to aerobic respiration
A.glucose →\rightarrow lactatelower
B.glucose →\rightarrow lactate + carbon dioxidelower
C.glucose →\rightarrow lactatehigher
D.glucose + oxygen →\rightarrow carbon dioxide + waterhigher

Question 12

MediumPaper 1A · calculator1 mark

Hydrogen carbonate indicator is red at atmospheric CO2\text{CO}_2 levels. It turns yellow when CO2\text{CO}_2 levels increase and purple when CO2\text{CO}_2 levels decrease. Tests were carried out in sealed tubes containing this indicator, varying the presence of an aquatic plant (Cabomba caroliniana) and a freshwater snail (Lymnaea stagnalis).

Which condition would result in the indicator turning purple?

A. Plant kept in the light without a snail

B. Plant kept in the dark without a snail

C. Snail kept in the light without a plant

D. Plant and snail kept in the dark

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  • Command terms are consistently under-read. Students answer describe when the question said explain, so they give an account with no reasons and cap at half marks. The reverse also happens on outline, where a student writes an essay for a 2-mark summary and runs out of time.
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What does Cell Respiration cover in IB Biology?

This topic explores how cells release energy from carbon compounds to produce adenosine triphosphate (ATP). ATP is the energy currency of cells, used for processes like active transport, macromolecule synthesis, and cellular movement. Energy is released when ATP is hydrolysed to ADP and phosphate, and required to synthesize ATP from ADP and phosphate.

Is Cell Respiration SL or HL?

Both. SL and HL students study Cell Respiration, and HL goes further: C1.2.7 Role of NAD as a carrier of hydrogen, and oxidation by removal of hydrogen. Oxidation is electron loss, so removing hydrogen with its electron (dehydrogenation) oxidizes the substrate. Redox reactions involve both oxidation and reduction, and NAD is reduced when it accepts hydrogen.

How do I revise Cell Respiration for IB Biology?

Start from the core idea: this topic explores how cells release energy from carbon compounds to produce adenosine triphosphate (ATP). In the exam: at SL the word equations and the aerobic/anaerobic comparison are the two reliable items, and the comparison is usually a table in the mark scheme. At HL, the four stages and where each happens (cytoplasm, matrix, inner membrane) are asked repeatedly, and location marks are awarded separately from process marks. Then practise exam-style questions, easiest first, writing out every step of your working before you check it.

How does FourtyFive help me practise Cell Respiration?

FourtyFive has 12 Cell Respiration questions. Every answer you write is marked mark by mark, IB-style, and you see where each mark was won or lost. Every part has a hint, the AI tutor helps you through the step you are stuck on, and your Study Profile picks what to practise next.

Is FourtyFive free for Cell Respiration practice?

Yes. A free account gives you 50 marked answers a month, and you do not need a card to sign up.

Can I handwrite Cell Respiration answers on an iPad?

Yes. In the FourtyFive iPad app you write your working by hand with Apple Pencil, the way you would on paper, and it is marked the same way.

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